Fig. 4.
Treatment of Nec-1s inhibits cell death induced by RIPK1 K376R. a Ripk1+/+ and Ripk1K376R/K376R MEFs were treated with T, TS, TSZ, TC, TCZ for indicated times with or without Nec-1s as indicated. Cell viability was determined using the CellTiter-Glo kit. The data are represented as the mean ± SEM (n = 3). P values were determined by Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001). Source data are provided as a Source Data file. b Pregnant Ripk1K376R/+ females were fed with Nec-1s (50 mg/kg) or saline solution once per day since day 9.5 of gestation and sacrificed on day 13.5. Representative embryos with indicated genotypes were presented. c Representative H&E staining (scale bars, 1 mm) and d TUNEL staining of fetal livers from mouse embryos of the indicated genotypes. Each image is representative of at least three embryos. Scale bars, 50 μm. e Quantification of TUNEL positive cells in Fig. 4d. The results are represented as ± SEM of three embryos per group. P value was calculated by Student’s t-test (***p < 0.001). Source data are provided as a Source Data file. f The fetal liver samples from three embryos per group were analyzed by western blot and immunoblotted with PARP, CC3, p-RIPK1(S166), and RIPK1