Fig. 7.
Embryonic lethality of Ripk1K376R/K376R mice is fully rescued by ablation of Fadd and Ripk3/Mlkl. a Representative photographs of mice with the indicated genotypes. b Predicted and observed frequencies in offspring from crosses of animals with the indicated genotypes. c Representative H&E-stained tissues from 12-week-old animals of the indicated genotypes. Results are representative of three mice of each genotype (Scale bars, 100 μm). d Representative lymphoid organs removed from 12-week-old mice of indicated genotypes. e Different cell subsets from 12-week-old animals were determined by flow cytometry using following markers: B cells (CD19+ or B220+), T cells(CD3+), Monocytes and macrophages(CD11b+), Granulocytes(Gr-1+). Data are represented as mean ± SEM (n = 3/genotype). P values were determined by Student’s t-test (**p < 0.01, ***p < 0.001). f Percentages and cell numbers of CD3+B220+ lymphocytes in spleen and lymph nodes from 12-week-old mice of indicated genotypes. Data are represented as mean ± SEM (n = 3/genotype). P values were determined by Student’s t-test (**p < 0.01, ***p < 0.001). Source data are provided as a Source Data file