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. 2019 Sep 13;9:13231. doi: 10.1038/s41598-019-49626-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Detection of surface-bound PAPP-A by flow cytometry. Data are geometric mean (±SEM) of the log fluorescence intensity measured on a flow cytometer. Data are presented as mean values of the fluorescence intensity measured from HEK293T cells incubated with supernatants containing either the serine variant (rPA_1144(Ser): red bars) or the tyrosine variant (rPA_1144(Tyr): blue bars) of the rs7020782 PAPP-A SNP, or with supernatants with no PAPP-A (MOCK: black bars). Experiments were completed in triplicate and tested with two different primary antibodies against PAPP-A (Upper panel: PA6 antibody and lower panel: mAb 1/41 antibody). No significant difference (ns) was observed between the two PAPP-A variants.