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. 2019 Sep 13;9:13269. doi: 10.1038/s41598-019-49516-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Serum IL-23p19 and IL-17A levels in R. anatipestifer-infected ducks. Two-week-old ducks were challenged intramuscularly with 5 × 10⁷ CFU of R. anatipestifer serotype 7 in 200 µl phosphate buffer saline (PBS). The uninfected control birds (NC) were administered 200 µl PBS intramuscularly. IL-17A and IL-23p19 levels in serum obtained at 24 h after infection were determined using the duck IL-17A and chicken IL-23 ELISA kits, respectively. COS-7 cells were transiently transfected with duIL-23p19-MYC construct. Cell lysates were collected after 48 h and used as positive control for chicken IL-23 ELISA. The results from one representative experiment of two independent experiments are shown. Data are shown as the mean ± SE (n = 10). *P < 0.05. RA, sera from R. anatipestifer-infected ducks.