Table 2.
Samples collected by ultrasound-guided fine-needle aspiration (US-FNA).
| Dog ID | Sample ID | Reproductive status | Number of US-FNA procedures | Cellularity of smears | Diagnosis | Blood Scoring | RNA (ng/µl) | RIN | RNA-Seq |
|---|---|---|---|---|---|---|---|---|---|
| 1* | US-1 | m | 1 | S | PCa | — | N/A | — | — |
| 2** | US-2 | n | 3 | S | PCa | — | N/A | — | — |
| US-3 | 4 | 59 | N/A | y | |||||
| 3 | US-4 | n | 1 | S | PCa | 2 | 44.1 | 6.9 | y |
| 4 | US-5 | m | 1 | S | nm | 0 | 2.3 | — | — |
| 5 | US-6 | n | 2 | S | PCa | 1 | 9.1 | N/A | — |
| 6 | US-7 | m | 2 | S | nm | 3 | 35.6 | 6.2 | y |
| 7 | US-8 | m | 3 | S | nm | 4 | 37.5 | — | — |
| 8 | US-9 | m | 2 | S | nm | 4 | 91 | — | — |
| 9 | US-10 | n | 2 | S | PCa | 3 | 21.7 | 1.9 | — |
| 10 | US-11 | m | 1 | S | nm | 3 | 33.2 | — | — |
| 11 | US-12 | m | 1 | S | nm | 4 | 85 | — | — |
| 12 | US-13 | m | 1 | S | nm | 2 | 90 | 6 | y |
| 13 | US-14 | m | 1 | S | nm | 3 | 54 | — | — |
| 14 | US-15 | m | 3 | S | nm | 4 | 61 | 6.1 | y |
| 15 | US-16 | m | 1 | S | nm | 3 | 74 | 5.7 | — |
| 16 | US-17 | m | 1 | Nd | N/A | 3 | 69 | — | — |
| 17 | US-18 | m | 1 | S | nm | 2 | 66 | — | — |
| 18 | US-19 | m | 2 | S | nm | 3 | 20.9 | — | — |
| 19 | US-20 | m | 2 | S | nm | 3 | 41.1 | — | — |
| 20 | US-21 | m | 1 | S | nm | 2 | 11.2 | 1 | — |
| 21 | US-22 | m | 2 | S | nm | 2 | 8.2 | — | — |
| 22 | US-23 | m | 1 | Nd | N/A | 3 | 18.7 | — | — |
| 23 | US-24 | m | 1 | S | nm | 0 | 26.6 | — | — |
| 24 | US-25 | m | 1 | S | nm | 3 | 54 | — | — |
| 25 | US-26 | m | 1 | S | nm | 2 | 30.1 | 4.2 | — |
| 26 | US-27 | m | 1 | S | nm | 3 | 56 | — | — |
| 27 | US-28 | m | 1 | S | nm | 4 | 47.8 | N/A | — |
| 28 | US-29 | m | 1 | S | nm | 2 | 30 | — | — |
| 29 | US-30 | m | 1 | S | nm | 3 | 72 | — | — |
| 30 | US-31 | m | 1 | Nd | N/A | 0 | N/A | — | — |
| 31 | US-32 | m | 1 | S | nm | 3 | 60 | 6.7 | y |
| 32 | US-33 | n | 1 | Nd | N/A | 2 | 11.3 | 1.5 | — |
| 33 | US-34 | m | 1 | S | nm | 2 | 51 | — | — |
| 34 | US-35 | m | 1 | S | nm | 2 | 68 | — | — |
| 35 | US-36 | m | 1 | S | nm | 3 | 378.6 | — | — |
| 36 | US-37 | m | 1 | S | nm | 3 | 48 | — | — |
| 37 | US-38 | m | 1 | S | nm | 3 | 24 | 3.2 | — |
| 38 | US-39 | m | 1 | S | nm | 3 | 99 | 5.7 | — |
| 39 | US-40 | m | 2 | S | nm | 4 | 601.1 | 7.2 | y |
| 40 | US-41 | n | 1 | Nd | N/A | 3 | 98 | — | — |
| 41 | US-42 | m | 3 | S | nm | 3 | 72 | — | — |
| 42*** | N/A | m | — | — | — | — | — | — | — |
| 43*** | N/A | m | — | — | — | — | — | — | — |
Sample details, including: identification number (ID) of the dogs and the corresponding sample ID; reproductive status, male (m) and neutered (n); cytological smears, sufficient (S) and non-diagnostic (Nd); diagnosis, prostate carcinoma (PCa) and non-malignant (nm); semi-quantitative scoring of blood contamination as follows: 0 = no, 1 = minimal, 2 = weak, 3 = moderate, 4 = strong blood contamination; RNA quantity; RNA integrity number (RIN); and samples used for RNA-Seq.
*Dog euthanised due to prostate carcinoma enabled additional sampling opportunity.
**Dog euthanised due to prostate carcinoma enabled additional sampling including one additional ultrasound-guided fine-needle aspiration.
***US-FNA procedure was omitted.
N/A, not available.
y, US-FNA samples selected for RNA-Seq.