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. 2019 Sep 11;21:100513. doi: 10.1016/j.ymgmr.2019.100513

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. S2 — Histologic characterization of cerebral organoids.

(A) Paraffin-embedded 4-week-old control cerebral organoid sectioned and stained with H&E. Scale bar, 250 μm. (B) Electron microscopy of 4-week-old control organoid shows columnar cells lining the ventricle-like structures, with cilia protruding into the ventricle space (arrows). Scale bars, 5 μm (left image); 1 μm (right image). (C) Representative immunofluorescence images of 4-week old control organoids stained for SOX2, a marker for neural progenitors (top panel) and SOX2/DAPI-merged staining (bottom panel). Scale bars, 500 μm. (D) Representative immunofluorescence images of 4-week old control organoids stained for GFAP, a marker for astrocytes (top panel) and GFAP/DAPI-merged staining (bottom panel). Scale bars, 500 μm.