Table 2.
List of antibodies
| Antibody/Agent | Dilution | Vendor | Order no. |
|---|---|---|---|
| Donkey secondary antibodies | 1:400 | Jackson Immunoresearch | |
| Streptavidin | 1:600 | Vector laboratories | SA-5649 |
| Endomucin | 1:400 | R&D Systems | AF4666 |
| F4/80 | 1:200 | AbD SeroTec | MCA497G |
| CD3 | 1:100 | BD Biosciences | 553308 |
| α Smooth muscle actin | 1:400 | Sigma | C6198 |
| VCAM-1 | 1:100 | BD Biosciences | 553330 |
| MPIO conjugation | |||
| VCAM-1 | BD Biosciences | 553330 | |
| IgG⁎,& | BD Biosciences | 553927 | |
| Blocking T-cell binding | |||
| CD49d#,& | BD Biosciences | 553313 | |
| CD49d#,& | BD Biosciences | 553154 | |
| CD49d | eBiosciences | 16–0492-82 | |
| VCAM-1 | GeneTex | GTX14360 | |
| VCAM-1 | eBiosciences | 16–0492-82 | |
| VCAM-1& | BD Biosciences | 553330 | |
| CD54 | Invitrogen | 16–0541-81 | |
| ICAM | R&D Systems | AF796 | |
| CD18#,& | BD Biosciences | 557440 | |
⁎
IgG antibodies used for in vivo blocking experiments, #In vivo blocking CD18/CD49d antibody mixture, &Antibodies used for Figure 4.