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. 2019 Sep 13;9:13254. doi: 10.1038/s41598-019-49684-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Alterations in FA and ceramide content in protein-bound epidermal lipids in Fatp4^−/− mice. Pooled fractions 4 and 5 (a) and fraction 3 (b) of protein-bound epidermal lipids of newborn mice were analyzed by ESI-MS as [M − H]⁻ ions in the negative-ion mode and quantified in arbitrary units. The decreased levels of nearly all OHFA species (a) and all saturated Cer(OS) species (b) seen in Fatp4^−/− mice were mostly normalized by the Fatp1 transgene. Data were obtained from 4 each of Fatp4^+/− (controls), Fatp4^−/−, and FATP1-rescued mutants with m/z ratios, standard deviation, and statistical significance shown (*P < 0.05; **P < 0.01; ***P < 0.001). See Materials and Methods for nomenclature.

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