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. 2019 Sep 13;9:13245. doi: 10.1038/s41598-019-49467-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Similarity of SEA-C2 plasmids to the pEC958 ST131 plasmid. From inner to outer circle, the following are indicated: GC content, GC skew, pTTSH10, pEC958, gene annotation, and labels of selected genes/loci. The rings representing pTTSH10 and pEC958 consist of a colored bar indicating the sequence identity to pTTSH16 (used as the reference). Areas with less than 50% identity are considered not present and indicated by the absence of a colored bar in that section of the ring, as indicated by the legend at the top right. Genes with certain functional annotations are indicated in color, as indicated by the legend at the bottom right. Gene names for resistance genes and plasmid replication genes are indicated; loci containing conjugation-related genes and clusters of replication genes are annotated with a colored line and a boxed label.