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. 2018 Dec 31;7(3):176–184. doi: 10.22088/IJMCM.BUMS.7.3.176

Table 1.

The sequence of primers used in RT-PCR

Gene Primers 5’ → 3’ GC% Tm ( o C) Amplicon size (bp)
FOXO1
NM_001191846
F: CGAGTGGATGGTGAAGAGTG
R: CGAATAAACTTGCTGTGTAGGG
55.00
44.50
55.00
54.90
114
FOXO3a
NM_001106359
F:CTCCCGTCAGCCAGTCTATG
R: GCTTAGCACCAGTGAAGTTCC
60.00
52.40
56.60
55.50
270
18S RNA
NM_046237.1
F: GTAACGCGTTGAACCCCATT
R: CCATCCAATCGGTAGTAGCG
54.80
54.40
64.50
64.20
151