Table 1.
Gene | Primers 5’ → 3’ | GC% | Tm ( o C) | Amplicon size (bp) |
---|---|---|---|---|
FOXO1
NM_001191846 |
F: CGAGTGGATGGTGAAGAGTG R: CGAATAAACTTGCTGTGTAGGG |
55.00 44.50 |
55.00 54.90 |
114 |
FOXO3a
NM_001106359 |
F:CTCCCGTCAGCCAGTCTATG R: GCTTAGCACCAGTGAAGTTCC |
60.00 52.40 |
56.60 55.50 |
270 |
18S RNA
NM_046237.1 |
F: GTAACGCGTTGAACCCCATT R: CCATCCAATCGGTAGTAGCG |
54.80 54.40 |
64.50 64.20 |
151 |