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. 2019 Sep 14;13:74. doi: 10.1186/s13036-019-0204-1

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Workflow of the expansion of hiPSCs as aggregates in VWBRs. Following 2D culture in tissue culture plates, hiPSCs were dissociated into single cells with Accutase and inoculated into the PBS MINI 0.1 bioreactor, where they formed aggregates. The culture was evaluated through daily sampling and analysis of the cells after culture