Fig. 3.

In vivo induction of REV-ERBα expression in Th17 cells suppresses EAE disease progression. EAE was induced in C57/BL6 mice by adoptive transfer of in vitro differentiated Rosa-M2rtTAxTRE-REV-ERBax2D2 transgenic Th17 cells. Recipient mice were treated with or without Doxycycline water (n = 7 per group) starting 2 d before Th17 cell adoptive transfer and were monitored for EAE disease progression. Mice were analyzed on day 24 after transfer. (A) Clinical scores of mice induced with EAE. (B) FACS analysis of IL-17A and IFN-γ production of transferred 2D2 CD4⁺ T cells infiltrating in the CNS tissues. (C) Representative H&E and Luxol Fast Blue staining of the spinal cords to show the sites of immune cell infiltration (filled arrow) and demyelination (open arrow). Data represents mean ± SEM. Statistical analyses were performed using 2-way analysis of variance (ANOVA) for EAE clinical score analysis and 2-tailed unpaired Student’s t test for other analysis, comparing the indicated groups (*P < 0.05, **P < 0.01).