Fig. 1.

Clec12A mediates optimal ISG expression. (A) Relative expression of Ifit3 and Irf7 in the thymi of whole body-irradiated mice. Data are depicted as the means ± SEM. Each data point represents 1 biological replicate. Representative data of 2 independent experiments are shown. (B) Relative expression of Ifit3 and Irf7 in WT BMDCs left untreated or stimulated with either MSU crystal or 3pRNA for 3 h. Data are depicted as the means of 3 mice per genotype ± SEM, with each individual data point shown. (C) Relative expression of Ifit3 and Irf7 in BMDCs left untreated or stimulated with 3pRNA alone or costimulated with 3pRNA+MSU crystal for 3 h. Data are depicted as the means of 3 mice per genotype + SEM. Representative data of 3 independent experiments are shown. (D–F) mRNA isolated from BMDCs treated as in C was subjected to RNA-seq. (D) Heatmap showing the expression profiles of Clec12a and putative ISGs that are among the top 100 most down-regulated genes in the costimulated Clec12a^−/− BMDCs versus WT BMDCs. Data shown are relative FPKM values of individual biological replicates in the RNA-seq. (E and F) Gene set enrichment analysis plots of the differentially expressed genes in the costimulated WT versus Clec12a^−/− BMDCs. The expression dataset was compared with MSigDB gene set collection C7 (immunological signature, 4,872 gene sets) (E) or collection H (Hallmark gene sets, 50 gene sets) (F). NES, normalized enrichment score; FDR, false discovery rate; AU, arbitrary unit. *P < 0.05, **P < 0.01, ***P < 0.001, n.s., not significant, 2-tailed t test (A and C), and 1-way ANOVA with post hoc Tukey’s test (B).