Fig. 5.

Single-cell RNA sequencing of neutrophil-depleted and neutrophil-intact primary sarcomas with and without radiation therapy. (A) Selected gene expression for genes in MAPK family signaling cascade pathway. Gene set derived from reactome database. (B) Gene set enrichment analysis for genes in MAPK signaling family cascades pathway shows enrichment in tumor cells from isotype + 20 Gy vs. anti-Ly6G + 20 Gy (P = 0.02). (C) Expressed genes within tumor cell clusters (0, 1, and 4) for tumors treated with anti-Ly6G + 20 Gy vs. isotype + 20 Gy. Axes represent gene expression level; differentially expressed genes are highlighted in red (anti-Ly6G + 20 Gy) or blue (isotype + 20 Gy).