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. 2019 Aug 27;116(37):18429–18434. doi: 10.1073/pnas.1905690116

Fig. 6.

Fig. 6.

SPG mutations suppress the arl-8 phenotype. (A) Schematic drawing of the DA9 neuron, showing the dorsal asynaptic region and the commissure that were observed and analyzed. The asterisk indicates the location where the commissure joins the dorsal nerve cord. (B–F) Representative images showing the localization of GFP::RAB-3 in WT (wt) (B), arl-8(wy271) (C), arl-8(wy271);unc-104(ADSPGV6M) (D), arl-8(wy271);unc-104(ARSPGA252V) (E), and arl-8(wy271);unc-104(ADSPGV6M)/+ (F). Integrated marker wyIs85 [Pitr-1::GFP::RAB-3] was used. Asterisks indicate the commissure bend shown in A. (Scale bars: 50 μm.) (G–I) Statistical analysis of mutant phenotypes. Shown are the length of the asynaptic region (G), the length of synaptic region in the dorsal axon (H), and the number of puncta misaccumulated at the commissure (I). Lines represent the median, and each dot represents 1 animal. Numbers above show actual median values. **Adjusted P < 0.01, ****adjusted P < 0.0001 compared with arl-8, Kruskal–Wallis 1-way ANOVA on ranks and Dunn’s multiple comparisons test. n = 30 animals for each genotype.