Fig. 1.

Os03g0122500 was the causal gene of Ef-cd. (A) NIL D248 headed earlier than its recurrent parent Shuhui881 (SH881). (B) Ef-cd was mapped in a 12.9-kb region. (C) Two T-DNA insertion mutants of Os03g0122500 headed later than the wild-type Hwayoung. (D) The insertion occurred in the first intron of Os03g0122500 in mutant ef-cd-1 and in the promoter region of Os03g0122500 in mutant ef-cd-2. The triangle represents the T-DNA insertion. The types of the binary vectors used for constructing the T-DNA insertion mutants are above the triangles. (E) Two RNAi lines of Os03g0122500 headed later than their wild-type Nipponbare. (F) Expression levels of Ef-cd and OsSOC1 were significantly decreased in the RNAi lines compared to their wild type. The P value was calculated using Student’s t test. **P < 0.01. (Scale bars, 20 cm.)