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. 2019 Aug 27;116(37):18655–18663. doi: 10.1073/pnas.1907833116

Fig. 1.

Fig. 1.

RmpA enhances expression of the K1 and K2 glycans in E. coli CLM37. (A) The K. pneumoniae K1 CPS gene map and (B) the K. pneumoniae K2 CPS gene map. Black arrows indicate UDP-sugar biosynthesis genes, purple arrows indicate CPS regulatory/surface export genes, orange arrows indicate transport/polymerase genes, blue arrows indicate glycosyltransferase genes, and green arrows indicate initiating glycosyltransferase genes. (C) Silver staining and Western blot analysis of LPS extracted from CLM37, CLM37 expressing the K1 locus, or CLM37 coexpressing the K1 locus and RmpA. (D) Silver staining of LPS extracted from CLM37, CLM37 expressing the K2 locus, or CLM37 coexpressing the K locus and RmpA.