Skip to main content
. 2019 Aug 26;116(37):18561–18570. doi: 10.1073/pnas.1905221116

Fig. 6.

Fig. 6.

Cdk2 inhibition and miR-199 overexpression improve zebrafish survival during systemic bacterial infection or sterile inflammation. (A) Survival of zebrafish larvae after infection of 1,000 CFU of PAK administered intravenously (i.v.) in the vector or miR-199 zebrafish line. Vector lines were left uninfected or injected with PBS as a control. (B) Survival of zebrafish larvae after infection of 1,000 CFU of PAK administered i.v. in the Cdk2 WT or D145N zebrafish line. (C) Survival of zebrafish larvae treated with vehicle, NU6140 (100 μM), or ApH (150 μM/20 mM) or left untreated after infection with 1,000 CFU of PAK administered i.v. (D) Survival of zebrafish larvae treated with indicated dilutions of roscovitine infected with 1,000 CFU of PAK administered i.v. (E) Survival of zebrafish larvae after injection of 25 ng of LPS administered i.v. in the vector or miR-199 zebrafish line. (F) Survival of zebrafish larvae treated with indicated dilutions of roscovitine injected with 25 ng of LPS i.v. In A–F, 1 representative experiment of 3 independent experiments (n ≥ 20 each group) is shown, using the Gehan–Breslow–Wilcoxon test.