Fig. 3.

Roquin knockdown results in increased expression of proinflammatory cytokines. (A) Heatmap of (log₂TPM) immune-related gene expression at the indicated time points in siCon or siRoq cells. (B) HFF cells were treated with siCon or siRoq, infected with HCMV (MOI = 2), and harvested at the indicated time points. mRNA levels of immune-related genes were measured by qRT-PCR. (C) Cytokine levels from cell-culture supernatant were measured by multiplex cytokine bead assay. (D) After siRNA transfection and HCMV infection (MOI = 2), cell culture supernatant (conditioned media; CM) was harvested at 72 hpi and UV-irradiated to inactivate viral particles. Naïve HFFs were incubated with CM, infected with HCMV (MOI = 0.5), and harvested at 72 hpi. UL99 mRNA level was measured by qRT-PCR. (B–D) Data represent mean ± SEM, n = 3; *P < 0.05; **P < 0.01; ***P < 0.001 according to two-tailed Student’s t test; NS, not significant.