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. 2019 Aug 26;116(37):18619–18628. doi: 10.1073/pnas.1909314116

Fig. 5.

Fig. 5.

IRF1 is a primary target of Roquin. (A) Relative expression of viral late gene (UL146) measured in HFFs transfected with indicated siRNA, infected with HCMV (MOI = 2), and harvested at 72 hpi. Cells were transfected with siCon, siRoq, or cotransfected with siRoq and siRNAs targeting Roquin-binding targets (siTarget). (B) Roquin-specific CLIP reads mapped to IRF1 mRNA and its predicted secondary structure. (C) RNA IP results confirming Roquin binding to IRF1 mRNA (mean ± SEM, n = 3). (D) IRF1 protein levels measured by immunoblot from HFF cells treated with siCon or siRoq and infected with HCMV (MOI = 2).