Fig. 3.

Number and phenotype of PLP_11–19–specific peripheral CD4 T cells in Plp1^KO or Plp1^WT mice expressing a fixed TCRβ chain. (A) Flow cytometry of pooled spleen and lymph node cells after enrichment of PLP_11–19–specific CD4 T cells, gated on CD4⁺CD8⁻Dump⁻ cells. The calculated mean number ± SEM of tetramer-positive cells/mouse is indicated (n ≥ 11 each). The graph on the right shows a summary. Each data point represents an individual mouse. (B) Foxp3^GFP and CD25 expression in tetramer-positive cells from pooled spleen and lymph node cells. Numbers indicate the mean frequency ± SEM of Foxp3⁺CD25⁺ cells (n ≥ 11 each). Data are summarized on the right. (C) FR4 and CD73 expression on gated Foxp3⁻ PLP_11–19–specific CD4 T cells from pooled spleen and lymph node cells. The dot plot on the right shows FR4 and CD73 expression in total Foxp3⁻ peripheral CD4 T cells for comparison (n ≥ 6 each).