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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: Cytotherapy. 2019 Jul 4;21(8):840–855. doi: 10.1016/j.jcyt.2019.06.008

Figure 5. Identification of novel HLA class II-restricted NS1 epitopes.

Figure 5.

Epitope mapping for NS1 protein was performed in five donors and results from one representative donor is shown. (A) A ZIKV-specific T-cell product from a ZIKV-exposed donor were stimulated with an NS1 peptide library pooled into 26 pools and the T-cell responses were measured by IFN-γ ELISpot. (B) Peptide matrix for NS1. NS1 peptides were divided into 26 mini-pools such that each peptide is present in two pools. Responses to pools 5 and 13, 5 and 23, 8 and 13, 8 and 23, 9 and 13, and 9 and 23 may be induced by individual peptides 5, 8, 9, 125, 128, and 129, respectively. (C) Testing of these single peptides by IFN-γ ELISpot revealed recognition of the single peptides of 5, 128, and 129 (mean ± SEM). (D) Blocking experiments with HLA class I and II blocking antibodies by IFN-γ ELISpot (mean ± SEM).