Fig. 8. TRE regulates Akt signal protein and the AMPK-SKP2-CARM1 signaling pathway.

a, c The expressions of Akt, p-Akt and AMPK-SKP2-CARM1 signaling pathway-related protein AMPK, p-AMPK, FOXO3a, p-FOXO3a, SKP2 and CARM1 in the Control, SUC and TRE groups as assessed by Western blotting. The gels have been run under the same experimental conditions, and cropped blots are used here. b, d Optical density values of Akt, p-Akt, AMPK, p-AMPK, FOXO3a, p-FOXO3a, SKP2 and CARM1 expressions in each group. e Nuclear CARM1-TFEB complex was detected by co-IP in the Control, SUC and TRE groups. f Optical density values of TFEB and CARM1 expressions in each group were quantified and analyzed. Values are expressed as means ± SEM, n = 6 per group. *p < 0.05 and **p < 0.01, vs. Control group; ^#p < 0.05 and ^##p < 0.01, vs. SUC group