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. 2019 Sep 2;2019:9719618. doi: 10.1155/2019/9719618

Figure 4.

Figure 4

Intracellular glutathione (GSH) and oxidized glutathione (GSSG) concentrations. IPEC-J2 cells were treated with DMEM-H medium (control) or with 70 μM paraquat (PQ) or 1.9 μM specific Nrf2 inhibitor (ML385) for 12 h. The concentrations of GSH and GSSG were determined using a GSH and GSSG assay kit from Beyotime Institute of Biotechnology. The ratio of GSSG to GSH was calculated. Data expressed as means ± SEM; n = 6 independent experiments. Different letters (A, B, and C) indicate significant differences (p < 0.05).