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. 2019 Sep 2;2019:3201062. doi: 10.1155/2019/3201062

Figure 9.

Figure 9

LPS induces enrichment of p300, HAT1, H3K27ac, and H3K9ac proteins at the proximal promoter region of Nox5 gene in cultured human Mac. Representative ChIP assay followed by agarose gel electrophoresis analysis of PCR products shows that p300 and HAT1 proteins are constitutively located within Nox5 gene promoter in resting Mac in vitro (a). Schematic depiction of the human Nox5 gene promoter (chromosome 15q23) showing the relative positions of the oligonucleotide primer sets spanning the entire proximal promoter region. Positive or negative DNA–p300/HAT1/H3K27ac/H3K9ac interactions are indicated with +/- marks (b). The increased abundance of p300, HAT1, H3K27ac, and H3K9ac proteins within Nox5 gene promoter in LPS-activated human Mac (c, d). n = 3; ∗∗∗P < 0.001. P values were taken in relation to resting Mac condition.