Table 1:

Cardiotoxicity screening phenotypes evaluated in this study.

Phenotype	Description
Functional
Peak frequency (beats per minute)	The average peak frequency, in beats per minute (BPM)
Decay To rise time ratio	The average ratio between the decay time and the rise time of the calcium flux (used as the in vitro surrogate for the QT interval)
Peak amplitude CV%	The coefficient of variation between the peak amplitudes
Peak spacing CV%	The coefficient of variation between the spacing of the peaks (used as a measure of irregular beating)
Cytotoxicity
Total cells	Total number of nuclei (cell count)
All nuclei mean integrated intensity	The total pixel intensity of the nuclear stain over the nuclear area, divided by the total number of cells
Positive W2 mean stain area	The average stained area (nucleus, cymiddlelasm, or both as defined in the W2 settings) for all cells scored as positive for W2 (in μm2)
Positive W2 mean stain Integrated intensity	The total pixel intensity of the W2 stain over the stained area in W2 positive cells, divided by the number of cells positive for W2
Positive W2 mean stain area/total cells	The average stained area (nucleus, cymiddlelasm, or both as defined in the W2 settings) for all cells scored as positive for W2, divided by the total number of cells