Figure 5.

A High-Activity NPR-1 Variant Partially Restores Stress-Induced Sleep to the Polymorphic Hawaii Strain

(A–C) Animals were exposed to SIS triggers as indicated and examined for locomotor quiescence as described in methods. Compared with the laboratory N2 strain, the Hawaii (HW) wild isolate fails to engage in locomotor quiescence during SIS. Locomotor quiescence is restored partially by introgression of the N2 NPR-1 genomic region into the Hawaii background (strain CX11400). Each data point represents the fraction of immobile animals in one trial of 25 young adult animals. Mean and SEM of three independent trials are shown. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns, not significant versus N2 (unless otherwise indicated by connecting bars on graphs), one-way ANOVA with Tukey's multiple comparisons test.