Figure 6.

Increased Quiescence Is Observed Following Oxygen-Induced SIS Deprivation

(A and B) Animals were exposed to UV light, placed into a Hammond chamber, and exposed to low oxygen (10%) as described in methods to allow animals to engage in SIS. One of the two plates was removed from the chamber and exposed to 21% oxygen (yellow shading) for sleep deprivation, followed by return to low oxygen (sleep-permissive) conditions. In npr-1(lf) mutant animals (A) and the Hawaiian wild isolate (B), sleep-deprived animals show increased locomotor quiescence relative to control animals. Mean and SEM of five independent trials of 25 animals each are shown. *p < 0.05, **p < 0.01, sleep-deprived versus control, Student's t test.