Dietary sulfur amino acid restriction contributes to the metabolic effects of DR. Mice were subjected to 4 weeks of ad libitum diet, dietary restriction (DR), or DR plus methionine (DR + Met), cysteine (DR + Cys), or casein (DR + Cas) supplementation (N = 6 per group). A = Body weight. B = Tissue weight at sacrifice. SQ (subcutaneous inguinal adipose tissue), BAT (brown adipose tissue), EPI (epididymal adipose tissue), Liver and Gastro (gastrocnemius muscle). C = Serum insulin. D = Serum leptin. E = Serum triglycerides. F = Blood glucose levels in animals fasted for 12 h (N = 6 per group). G = glucose tolerance test performed after 12 h fasting. H = serum adiponectin. Results are presented as mean ± SEM. p < 0.05: a, compared to others groups; b, compared to the ad libitum (AL) group; c, compared to the DR group. One-way ANOVA test with Tukey's post-test.