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. 2019 Aug 2;38(18):e101426. doi: 10.15252/embj.2018101426

Figure EV1. Hormone induces the expression of C/EBPα in breast cancer cells.

Figure EV1

  1. Time kinetics of C/EBPα expression after hormone induction in T47D cells. T47D cells treated with ethanol or with 10 nM R5020 as indicated were lysed and analyzed by immunoblot with α‐C/EBPα (left panel) or with α‐C/EBPβ (right panel). An antibody to PARP was used as loading control.
  2. Quantitation of the bands shown in panel (A) using densitometric analysis and ImageJ software. Values were normalized to time zero (T0). Each bar represents the mean ± SD from two experiments.
  3. T47D cells transfected with control or C/EBPα siRNAs and treated or not with 10 nM R5020 as indicated were lysed and analyzed by immunoblot with specific antibodies for PR, ER, and C/EBPα.
  4. GO analysis of C/EBPα‐dependent genes in T47D cells. We used g:Profiler (Reimand et al, 2016) to identify Gene Ontology Biological Process (GO:BP) in the differentially expressed genes.

Source data are available online for this figure.