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. 2019 Aug 2;38(18):e101426. doi: 10.15252/embj.2018101426

Figure EV4. The interactome of C/EBPα reveals a preferential interaction with SWI/SNF, NURD, KDM1A/HDAC/REST, and RSF chromatin remodeling complexes.

Figure EV4

  1. Immunoprecipitation of C/EBPα followed by mass spec in a T47D cell line expressing inducible C/EBPα (see Materials and Methods). Lower panels: Analysis of the peptides showed that the interacting proteins function in chromatin remodeling, chromatin modification, mitotic cell cycle, DNA conformation change, DNA repair, and nucleosome organization. Right panel: By using the CORUM database (Ruepp et al, 2008), we identify SWI/SNF (BAF), NURD, and CHD complexes as well as histone deacetylases.
  2. Analysis of the ChIP‐seq of P300 in T47D cells treated or not with hormone in “assisted”, “induced shared”, “PR‐exclusive”, “C/EBPα‐constitutive”, and “C/EBPα‐exclusive” regions.
  3. Validation of the C/EBPα interactors by co‐IP in T47DindC/EBPα cells −/+ dox (left panel) and T47D cells −/+ hormone (right panel). The immunoprecipitates (IP) were analyzed by immunoblotting with specific antibodies for C/EBPα, Topo 2α, BRG1, BAF170, SNF2h, CHD4, KDM1, CBX3, and PR.
  4. T47D cells treated or not with 6 and 12 μM of ICRF193 and 10 nM R5020 as indicated were subjected to flow cytometric analysis of cell cycle to quantify the cells in S phase. Each value corresponds to the mean ± SD of three experiments performed in duplicate. **P < 0.01, *P < 0.05 using Student's t‐test.

Source data are available online for this figure.