Figure 6. TRAF3IP3 mediates interferon response to virus infection in BMDMs and PEMs from mice.

• A–E
  BMDMs from wild‐type, Traf3ip3 ^−/−, and Mavs ^−/− mice were treated with or without VSV, SeV, poly (I:C), or HSV‐1, respectively, as indicated for 6 h. Ifnb (A), Ifna4 (B), Il6 (C), Isg54 (D), and Cxcl10 (E) induction was measured, respectively, by qPCR. All data are presented as the mean values based on three independent experiments, and error bars indicate s.d. P values were determined by unpaired two‐tailed Student's t‐test. *P < 0.05 and **P < 0.01. NS indicates no statistically significant difference.
• F
  BMDMs from wild‐type, Traf3ip3 ^−/−, and Mavs ^−/− mice were infected with or without VSV for 6 h. Cells were then collected and following subcellular fractionation, S5 fractions were subjected to native‐PAGE to examine IRF3 dimer, and P5 fractions contained mitochondria were subjected to SDD‐AGE to examine MAVS aggregation.

Source data are available online for this figure.