Figure 7. Traf3ip3 deficiency attenuates antiviral immunity in mice.

• A–F
  Wild‐type, Traf3ip3 ^−/−, and Mavs ^−/− mice (three littermates per group) were infected intravenously with VSV at a MOI of 2 × 10⁸. The spleens were collected 4 h or 8 h after infection, and Ifna4 (A), Ifnb (B), Isg54 (C), and Il6 (D) inductions were measured, respectively, by qPCR. VSV RNA levels were measured by qPCR (E). The sera were collected from the treated mice and used for the measurement of IFNβ production by ELISA (F). All data are presented as the mean values based on three independent experiments, and error bars indicate s.d. P values were determined by unpaired two‐tailed Student's t‐test. *P < 0.05 and **P < 0.01. NS indicates no statistically significant difference.
• G
  Wild‐type, Traf3ip3 ^−/−, and Mavs ^−/− mice (n = 6 each) were injected with VSV via tail vein injection at 5 × 10⁷ pfu per mouse, and the survival rates were monitored for 15 days. P values were determined by unpaired two‐tailed Student's t‐test. *P < 0.05.