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. 2019 Aug;39:101497. doi: 10.1016/j.scr.2019.101497

Fig. 5.

Fig. 5

BMP2 had lower biological activity but gave higher chondrogenic associated gene expression and phosphorylation of SMAD proteins in hESC chondrogenesis. (a) Alkaline phosphatase assay of SW1353 cells, were treated with different concentrations of BMP2 and BMP4 for three days. (b) Protocol overview of BMP2 and BMP4 treatments to induce chondrogenesis. (c) QRT-PCR gene expression analysis of chondrogenic and pluripotency associated genes after BMP4 or BMP2 treatment on MAN7 cells. All data are shown as relative expression compared to GAPDH mean ± SEM (n = 3). One way-ANOVA was performed. ⁎⁎⁎P < 0.0005. ⁎⁎⁎⁎P < 0.0001. (d) Representative flow cytometry data for protein expression of SMAD1/5/9 and p-SMAD1/5/9 after BMP4 or BMP2 treatment, on chondrogenic cells generated from MAN7. (e) Western blot analysis of SMAD1/5/9 and p-SMAD1/5/9 in day10 cells treated with BMP2 or BMP4 for one-hour. Protein expression shown as relative to GAPDH.