Representative scheme of T6SS sheath dynamics in wild type. T6SS sheath stays extended or presumably anchored to the membrane via TagA for up to 220 s before contraction (average anchored time: 50 s, n = 150).
Representative scheme of T6SS sheath dynamics in the tagA mutant. Sheath does not stay extended before contraction.
Representative kymograph of VipA‐mCherry2 and TssA‐mNeonGreen dynamics in a tagA mutant strain background. 30% of all sheath structures show TssA‐mNeonGreen signal at distal end (white arrows) after contraction.
Quantification of number of sheath contractions. 0.6 sheath contractions per minute per cell were measured in WT and 0.2 contractions per minute per cell in the ∆tagA mutant.
Sheath length significantly increases in the ∆tagA mutant. * Samples are significantly different from each other (two‐sample t‐test, P < 0.001).
Data information: In boxplots shown here, the central mark of each box indicates the median and the bottom and top edges of the box indicate the 25
th and 75
th percentiles, respectively. The whiskers extend to the most extreme data points not considered outliers, and the outliers are plotted individually using the “+” symbol labeled in red.
