Figure 3.

H&E Staining of Tsc1-Floxed Brains

(A–F) Coronal section of the fourth (4^th V) (A–C) and third ventricles (3^rd V) (D–F). (A and D) Coronal sections of normal non-injected Tsc1-floxed brains at P37 showed normal-sized ventricles (A) and the subependymal layer (D). (B and E) Tsc1-floxed mice injected i.c.v. with AAV1-Cre on P0 exhibited enlarged ventricle, indicating incipient hydrocephalus (B) and cell clumping, indicating overgrowth of subependymal cells lining the ventricle (E, inset: arrowheads showing the higher magnification of clumping of subependymal cells). (C and F) Tsc1-floxed mice injected i.c.v. with AAV1-Cre on P0, then retro-orbitally injected with AAV9-hamartin at P21 showed normal ventricular size (C) and a single subependymal layer of cells surrounding the ventricles (F). Original magnification, ×10. Scale bars, 200 μm.