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. 2019 Aug 16;15:18–26. doi: 10.1016/j.omtm.2019.08.003

Figure 4.

Figure 4

Ki67 Immunostaining of Tsc1-Floxed Brains

(A–C) Tsc1-floxed mice injected with AAV1-Cre vectors only show more proliferating cells adjacent to the lateral ventricular area (B) as compared with the naive group (A) and the Tsc1-floxed mice rescued by AAV9-hamartin vectors (C). Yellow arrows indicate the proliferating Ki67+ve cells some distance away from the ventricular layer. The corresponding brain sections were counterstained with DAPI (D–F). Original magnification, ×20. Scale bar, 100 μm. CC, corpus callosum; LV, lateral ventricle.