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. Author manuscript; available in PMC: 2019 Sep 16.
Published in final edited form as: J Vis Exp. 2018 Nov 28;(141):10.3791/58794. doi: 10.3791/58794

Figure 2: Imaging chamber, zebrafish mounting and heart injection procedures, and needles.

Figure 2:

(A) Shown is an imaging chamber with a larva (outlined by a dashed rectangle) pinned to the center atop the silicone encapsulant. (B1) Shown is a 5 dpf larva immobilized by two pins. A large head pin is placed perpendicular to the body just posterior to the eye. The two eyes are completely superimposed so the bottom eye is entirely obscured by the upper eye. A small tail pin intersects the notochord in the tail. The larva is flat and not twisted. (B2) To paralyze larva, a heart injection needle is oriented perpendicular to the body and brought adjacent to the heart. The heart injection needle should contact the pigment cell in front of the heart. (B2′) Depression of the needle into the skin causes indentation of the pigment cell in front of the heart. (C) Needles in order from left to right: example of a heart injection needle with an opening of approximately 3 μm; example of a good fluid-jet needle with an opening of approximately 50 μm; example of a poorly broken fluid-jet needle that is large and jagged and will likely produce excessive and irregular stimuli.