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. 2019 Sep 16;17:96. doi: 10.1186/s12951-019-0531-x

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Sensitivity analysis of the blocking ELISA. A 96-well plate was coated with the PEDV N protein (100 ng/well) and then blocked. Serially diluted positive and negative sera were added to the wells and incubated at 37 °C for 2 h. Then, biNb2 was added to the wells, and HRP-conjugated streptavidin was added to the wells. Values are the mean PI (%) of three well replicated. The small figure in the upper right corner shows the OD_450nm value of the positive and negative sera. The dotted lines represent the upper and lower cutoff values