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. 2019 Jul 30;8(12):5651–5661. doi: 10.1002/cam4.2440

Figure 4.

Figure 4

The bioinformatics analysis, qRT‐PCR, western blot, and ChIP‐qPCR assay revealed that HOXA10 might induce BCL2 expression via binding to its promoter region. A, The “TF‐gene interactions” analysis of HOXA10 and BCL2 in the database NetworkAnalyst. B, A PPI network was built between HOXA10 and BCL2 with the database STRING. C, qRT‐PCR showed the relative BCL2 mRNA level in different cells with changed HOXA10 expression. D, Expression of BCL2, Bax, cleaved forms of Caspase‐9, Caspase‐3, and PARP in different GC cells with altered HOXA10 expression. E, HOXA10 binding motif acquired from JASPAR and the relative primer position within the BCL2 promoter region. F, ChIP‐qPCR assay indicated the possible positive HOXA10 binding sites across the BCL2 promoter region. G, 3% agarose gel electrophoresis of ChIP‐qPCR products. **P < .01, ***P < .001. ChIP‐qPCR, chromatin immunoprecipitation and quantitative PCR; PPI, protein‐protein interaction; qRT‐PCR, quantitative real‐time polymerase chain reaction; TF‐gene, transcription factor‐gene