Table 2.

Results of analyses of molecular variance (AMOVA) for Salix humboldtiana in central Mexico based on eight nSSRs loci with different population groupings: among and within all populations and grouped according to basin (Balsas/Lerma) and according to the results of the Bayesian clustering algorithms GENELAND and STRUCTURE

Grouping	df	R ST			F ST
		SS	VC	%	SS	VC	%
Basin
Among groups	1	44,098.3	354.7	28.9***	66.8	0.5	13.6***
Among populations within groups	10	75,334.8	368.5	30.1***	139.3	0.6	18.4***
Within populations	222	111,525.5	502.4	41	505.1	2.3	67.9
Total	233	230,958.6	1,225.5		711.2	3.3
Structure
Among groups	2	85,404.2	531.2	44***	113.6	0.6	18.7***
Among populations within groups	9	34,029	173.8	14***	95.2	0.4	12.9***
Within populations	222	111,525.5	502.4	42	509.2	2.3	68.4
Total	233	230,958.6	1,207.4		718.0	3.4
Geneland
Among groups	7	115,947.5	564.5	50.9***	178.8	0.7	21.38***
Among populations within groups	4	4,552.5	32.4	2.9***	27.3	0.2	7.37***
Within populations	222	112,631.4	513	46.2	505.2	2.3	71.25
Total	233	233,232.3	1,109.9		711.2	3.2
Populations
Among populations	11	120,499.9	543.7	51.8***	208.8	0.87	27.47***
Among individuals within populations	222	112,631.4	512.9	48.3	509.194	2.32	72.52
Total	233	233,131.3	1,063		711.2	3.1

Analyses were performed with both the infinite alleles (F _ST) and stepwise (R _ST) mutation models.

Abbreviations: df, degrees of freedom; SS, sum of squares; VC, variance components.

^*** p < .001.