Table 4.
The results of the Cox proportional hazards regression: unadjusted and adjusted effects of the studied SNP on overall survival in AML patients
| Factors | Levels/genotypes | Events (%) | Univariable analysis | Multivariable analysis‐reduced model | |||
|---|---|---|---|---|---|---|---|
| HRa (95% CI) | P‐value | HRb (95% CI) | Correctedc HR (95% CI) | P‐value | |||
| Gender | F | 79 (80.6) | Reference | .293 | NA | NA | NA |
| M | 91 (85.8) | 1.18 (0.87‐1.59) | |||||
| Age | <65 y | 109 (79.0) | Reference | <.001 | Reference | Reference | <.001 |
| ≥65 y | 61 (92.4) | 2.15 (1.55‐2.99) | 2.76 (1.96‐3.89) | 1.61 (1.37‐1.89) | |||
| TGF‐β1 rs1800470 | Wild‐type genotype | 11 (73.3) | Reference | .857 | NA | NA | NA |
| variant | 159 (84.1) | 0.95 (0.51‐1.75) | |||||
| IFN‐γ rs2430561 | Wild‐type genotype | 41 (82.0) | Reference | .705 | NA | NA | NA |
| variant | 129 (83.8) | 1.07 (0.75‐1.53) | |||||
| TNF‐α rs361525 | Wild‐type genotype | 161 (83.4) | Reference | .546 | NA | NA | NA |
| variant | 9 (81.8) | 1.23 (0.63‐2.41) | |||||
| TNF‐α rs1800629 | Wild‐type genotype | 111 (83.5) | Reference | .500 | Reference | Reference | .070 |
| variant | 59 (83.1) | 1.12 (0.81‐1.54) | 1.39 (0.99‐1.93) | 1.17 (1.00‐1.38) | |||
| TNF‐α rs1800750 | Wild‐type genotype | 166 (83.0) | Reference | .005 | Reference | Reference | .007 |
| variant | 4 (100.0) | 4.22 (1.54‐11.52) | 5.84 (2.07‐16.45) | 1.27 (1.10‐1.47) | |||
| IL‐6 rs1800795 | Wild‐type genotype | 24 (77.4) | Reference | .941 | NA | NA | NA |
| variant | 146 (84.4) | 0.98 (0.64‐1.52) | |||||
| IL‐10 rs1800872 | Wild‐type genotype | 88 (83.0) | Reference | .885 | NA | NA | NA |
| variant | 82 (83.7) | 1.02 (0.76‐1.38) | |||||
| IL‐10 rs1800896 | Wild‐type genotype | 52 (85.2) | Reference | .385 | NA | NA | NA |
| variant | 118(82.5) | 0.87 (0.62‐1.20) | |||||
| FLT3‐ITD status | Absent | 140 (81.9) | Reference | .009 | NA | NA | NA |
| Present | 30 (90.9) | 1.70 (1.14‐2.54) | |||||
| FLT3 D835 status | Absent | 160 (83.3) | Reference | .739 | NA | NA | NA |
| Present | 10 (83.3) | 0.90 (0.47‐1.70) | |||||
| NPM1 status | Absent | 142 (83.0) | Reference | .618 | NA | NA | NA |
| Present | 28 (84.8) | 1.11 (0.74‐1.66) | |||||
| DNMT3A R882 status | Absent | 149 (82.3) | Reference | .368 | NA | NA | NA |
| Present | 21 (91.3) | 1.23 (0.78‐1.95) | |||||
| Hgb level | <10 | 127 (82.5) | Reference | .217 | NA | NA | NA |
| ≥10 | 43 (86.0) | 0.80 (0.57‐1.14) | |||||
| Htc | >35 | 11 (100.0) | Reference | .851 | NA | NA | NA |
| ≤35 | 159 (82.4) | 0.94 (0.51‐1.74) | |||||
| PLT | <40 000 | 93 (84.5) | 1.40 (1.03‐1.89) | .039 | 1.39 (0.99‐1.85) | 1.17 (1.00‐1.38) | .075 |
| ≥40 000 | 77 (81.9) | Reference | Reference | Reference | |||
| Blasts (%) | <70 | 98 (79.0) | Reference | .325 | Reference | Reference | .042 |
| >70 | 72 (90.0) | 1.17 (0.86‐1.58) | 1.46 (1.06‐2.03) | 1.20 (1.03‐1.41) | |||
| LDH level (IU/L) | <600 | 68 (78.2) | Reference | .008 | Reference | Reference | .022 |
| ≥600 | 102 (87.2) | 1.52 (1.11‐2.06) | 1.54 (1.10‐2.16) | 1.24 (1.05‐1.47) | |||
| ECOG status | ≤1 | 18 (56.3) | Reference | .010 | NA | NA | NA |
| ≥2 | 152 (88.4) | 1.90 (1.16‐3.10) | |||||
| Cytogenetic risk (1) | Low‐risk | 17 (70.8) | Reference | .422 | NA | NA | NA |
| Intermediate | 98 (79.7) | 1.24 (0.74‐2.07) | |||||
| Cytogenetic risk (2) | Low‐risk | 17 (70.8) | Reference | .003 | Reference | Reference | .021 |
| High‐risk | 55 (96.5) | 2.29 (1.32‐3.97) | 1.54 (1.10‐2.16) | 1.34 (1.15‐1.56) | |||
| WBC count | <50 000 | 137 (82.5) | Reference | .077 | NA | NA | NA |
| ≥50 000 | 33 (86.8) | 1.41 (0.96‐2.07) | |||||
| AML type | 0 | 137 (82.0) | Reference | .266 | NA | NA | NA |
| 1 + 2 | 33 (89.2) | 1.24 (0.85‐1.82) | |||||
Events = number of deaths; % was calculated in relation to the number of cases corresponding to each category of the study.
Cytogenetic risk variable was transformed in two dummy variables in cox regression analysis
Abbreviations: 95% CI, 95% confidence interval; HR, hazard ratio; MIC, Minimizing approximated Information Criterion; NA, not selected to be included in the reduced model.
a
Crude HR.
b
Adjusted HR from unpenalized Cox PH regression model.
c
Adjusted HR from penalized Cox PH regression model estimated by MIC method.
Bold values denoted statistically significant results (P < 0.05).