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. 2018 Jun 8;2(3):e54. doi: 10.1097/HS9.0000000000000054

Figure 3.

Figure 3

Effect of LSD1 inhibition on lineage commitment and hematopoietic stem and progenitor populations in Jak2V617F mice. (A–H) BM cells were stained with antibodies against a cocktail of lineage markers as well as against c-kit, Sca-1, CD34, Fc-γR, and Flt3/Flk2. All given subpopulations, defined as previously described,51,52,55 are depicted as a percentage of total BM cells. (A) Lineage−, c-kit+, Sca-1+ (LSK) cells. (B) Long-term hematopoietic stem cell (LT-HSC: LSK, CD34neg, FLK2/FLT3neg). (C) Short-term hematopoietic stem cell (ST-HSC: LSK, CD34int, FLK2/FLT3neg). (D) Lineage−, c-kit+, Sca-1+, CD135−, CD90.1−, CD34lo cells. (E) Multipotent progenitor (MPP). (F) Common myeloid progenitor (CMP). (G) Granulocyte-macrophage progenitor (GMP). (H) Megakaryocyte-erythrocyte progenitor (MEP). (A–H) n = 6 to 7 per group, as indicated. Mean and standard error of mean are shown. Statistical analysis was conducted using Student t tests. ∗P < 0.05, ∗∗P < 0.01 versus vehicle-treated mice. BM = bone marrow.