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. 2019 Apr 24;3(3):e233. doi: 10.1097/HS9.0000000000000233

Figure 2.

Figure 2

GAS6 levels in MPN; A, Levels of GAS6 were measured in plasma of normal and JAK2 V617F patients by ELISA and the results expressed as ng/ml of plasma (mean ± SEM, n = 6). RNA expression levels in CD34+ cells were measured using Illumina HiSeq NGS and data shown as the number of normalized RNA reads observed. Results for housekeeping genes (B) and GAS6 (C) are shown (mean ± SEM, n = 3 normals, n = 7 for JAK2 V617F). D, CD34+ cells isolated from MPN patients and healthy controls were incubated with 20 μM DCFDA to assess ROS levels. Amalgamated flow cytometry data is displayed as median fluorescent intensity ± SEM (n = 3 normals, n = 7 for JAK2 V617F). The results of a t test are shown, ns indicates not significant.