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. 2019 Apr 24;3(3):e233. doi: 10.1097/HS9.0000000000000233

Figure 6.

Figure 6

BGB324 and ruxolitinib reduce STAT5 phosphorylation observed in PV patients; CD34+ cells isolated from JAK2 V617F patients were cultured in Fischer medium supplemented with 5% horse serum in the presence or absence of 3 μM BGB324 or 50 nM ruxolotinib for 4 hours and the effects on T180/Y182 phospho-p38MAPK (A–C), Y507 phospho-LYN (D–F) and Y694 phospho-STAT5a (G–I) analyzed on a Novocyte flow cytometer (ACEA Biosciences) using FloJo software. Representative FACS plots are shown (A, B, D, E, G, and H) and amalgamated data (C, F, and I) displayed as median fluorescent intensity ± SEM (n = 9 for STAT5, n = 5 for p38MAPK and LYN). The results of a t test are shown (NS = not significant, ∗ < 0.05).