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. 2019 Jun 1;3(3):e255. doi: 10.1097/HS9.0000000000000255

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Copyright © 2019 the Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the European Hematology Association.

This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc-nd/4.0

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GeneScan profiles of cases with discrepancies between IVS-1T-1F (left) and TRG-1T-1F (right) assays. Regarding case NL-088, the TRGV-TRGJ rearrangement was seen in both PCRs but the TRGV-TRGJP rearrangement was not detected with the TRG-1T-1F PCR, due to the absence of a TRGJP primer, in contrast to the assay in Figure 4. It is not possible to determine which peak corresponds to the TRGJP rearrangement in the IVS-1T-1F PCR, as the primer information is not provided in the IVS TRG V2.0 kit assay.