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. 2019 Aug 2;10(9):1296–1301. doi: 10.1021/acsmedchemlett.9b00227

Table 5. Viability of MM.1S Cells Treated with Compounds.

graphic file with name ml9b00227_0009.jpg

compound R X Y BRD4-D1 IC50 (μM)a MM.1S EC50 (μM)b
22 C(NH)NH2 O N 9.7 ± 1.8 >50
5 C(NH)NH2 O CH 3.6 ± 0.05, 1.7 ± 0.30c >50
27 C(NH)NH2 NH CH 4.5 ± 0.35 >50
28 H O N 11 ± 2.5 6.6 ± 1.9
29 H O CH 2.9 ± 0.07, 1.3 ± 0.25c 2.9 ± 0.6
30 H NH CH 3.9 ± 0.35 6.3 ± 1.1
a

IC50 values were determined by fluorescence anisotropy. Data represents the mean and standard deviation of three independent trials.

b

Data reported are mean ± SEM of three biological replicates, with three technical replicates each. EC50 values were determined using the Nonlinear fit algorithm on GraphPad Prism.

c

IC50 values were determined by AlphaScreen by Reaction Biology.