Figure 2.

Number of macrophages in the skeletal muscle and spleen of Dmd^mdx rats. Cytofluorimetry of single-cell suspensions from hind limb muscles or spleen of WT or Dmd^mdx (KO) rats at the indicated time points. (A) Total number of macrophages (CD68⁺ cells) per gram of muscle (upper left panel) or whole spleen (upper right panel). Representative dot plots of high granularity macrophages using side scatter (SSC^high) vs. CD68⁺ cells after gating on viable (negatively stained cells) CD45⁺ cells from muscle of WT or Dmd^mdx 12-week old rats (lower panel). (B) Total number of viable CD68⁺CD163⁺ type 2 macrophages per gram of muscle (upper left panel) or whole spleen (upper right panel). Representative dot plots of viable CD68⁺CD163⁺ cells from muscle of WT or Dmd^mdx 12-week old rats (lower panels). (C) Type 2 (CD68⁺CD163⁺) to type 1 macrophage (CD68⁺CD163⁻) ratios in muscle (left panel) or spleen (right panel) of WT (black) or Dmd^mdx (gray) rats. Data presented in (A–C) are given as mean ± SEM of 3, 6, 6, 7, and 8 (at 2, 4, 8, 12, and 16 weeks of age, respectively) Dmd^mdx rats and of 4, 6, 4, 3, and 4 (at 2, 4, 8, 12, and 16 weeks of age, respectively) WT rats. ^*p < 0.05 and ^**p < 0.01. Results were obtained from several experiments (n = 1, 2, 2, 3, and 3 at 2, 4, 8, 12, and 16 weeks of age, respectively) which were performed using all animal groups in each experiment.