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. 2019 Jul 25;10(19):4532–4539. doi: 10.7150/jca.30788

Figure 1.

Figure 1

Tumor inoculation procedure: A. Exposure the bladder; B. Cystotomy along the midline; C. 1 ml needle (containing 300 ul of cell suspension) punctures into the mucosa on the side wall of the bladder; D. After about 1.5cm of immersion in the submucosa of the bladder, the thumb of the left hand presses against point of the needle, and 300ul of cell suspension is injected under the mucosa; E. Blisters formed by cell suspension after injection; F. Close the bladder with absorbable suture.