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. 2019 Jul 25;10(19):4499–4508. doi: 10.7150/jca.31487

Figure 1.

Figure 1

Galangin inhibited cell proliferation, migration, invasion and downregulated CD44 and EMT markers in glioblastoma cells. (A) U87 and U251 cells were incubated with different concentrations of galangin for 24, 48 and 72 hours and then cell viability was detected using the CCK-8 assay. (B) U87 and U251 cells were treated for 24 hours with different doses of galangin, then the EdU assay was performed. (C, D) U87(C) and U251(D) cells were treated with the indicated doses of galangin for 24 hours, and transwell assay was applied to examine the migration and invasion. (E, F) U87 and U251 cells were treated with different doses of galangin for 24 hours, and qPCR (E) and Western blotting (F) were applied to analyze the mRNA and protein expression levels of CD44 and the EMT markers. n=3 or 4, and all tests were performed in triplicate. *P<0.05, compared with control (0 μM).